Structure of human nucleosome containing the testis-specific histone variant TSH2B

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Structure of human nucleosome containing the testis-specific histone variant TSH2B

The human histone H2B variant TSH2B is highly expressed in testis and may function in the chromatin transition during spermatogenesis. In the present study, the crystal structure of the human testis-specific nucleosome containing TSH2B was determined at 2.8 Å resolution. A local structural difference between TSH2B and canonical H2B in nucleosomes was detected around the TSH2B-specific amino-aci...

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Structural basis of instability of the nucleosome containing a testis-specific histone variant, human H3T.

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Five non-allelic histone H3 variants, H3.1, H3.2, H3.3, H3t and CENP-A, have been identified in mammals. H3t is robustly expressed in the testis, and thus was assigned as the testis-specific H3 variant. However, recent proteomics and tissue-specific RT-PCR experiments revealed a small amount of H3t expression in somatic cells. In the present study, we purified human H3t as a recombinant protein...

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ژورنال

عنوان ژورنال: Acta Crystallographica Section F Structural Biology Communications

سال: 2014

ISSN: 2053-230X

DOI: 10.1107/s2053230x14004695